Neomycin ELISA Test Kit

Neomycin ELISA test Kit
Catalog No. LSY-10005
1. Principle
This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained.
 
2. Technical specifications
Sensitivity: 0.5ppb
Incubation Temperature: 25℃
Incubation Time: 60min—15min
Detection limit
Tissue(Chicken/liver, pork/liver, shrimp, fish) 10ppb
Honey 10ppb
Milk, milk powder 10ppb
 Recovery rate
Tissue (chicken, pork, fish, shrimp), honey 85±19%
Liver (chicken or porcine) 70±17%
Milk, milk powder   85±16%
Cross-reaction rate
Neomycin 100%
Streptomycin < 0.1%
Gentamicin < 0.1%
Dihydrostreptomycin < 0.1%
Kanamycin < 0.1%
Tobramycin < 0.1%
Sisomicin < 0.1%
 

3. Components